IDENTIFICATION OF LACTIC ACID BACTERIA FROM BIOFLOK TECHNOLOGY WHICH HAS BEEN GAVE MOLLASES ON RED TILAPIA (Oreochromis sp.) AQUACULTURE

Nelli Karolina Sitorus, Iesje Lukistyowati, Henni Syawal, Iskandar Putra

Abstract


The aim of this research was to  determine the types of lactic acid bacteria from biofloc technology with the addition of mollase in red tilapia aquaculture containers and to determine the optimum growth of lactic acid bacteria at pH 2, 4, and 6, and also to observe the density of bacteria in the biofloc container water during maintenance. The methods used in this research are survey and experiments by taking flok from maintenance container and then identifying the lactic acid bacteria. The results of lactic acid bacteria inoculation obtained 16 isolates isolated from floc which can only grow at pH 4 and pH 6. Identification based on physical tests, chemical tests, and biochemical tests showed that the isolates were classified into Bacillus and Streptococcus. The amount of bacterial density water from containers of biofloc technology were given mollase during research reached the highest point in the third week, which averaged 109 CFU/mL.

Keywords


Identification; Lactic Acid Bacteria; Biofloc technology; red tilapia

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References


Azim, M.E., Little,D. North, B. (2007). Growth and Welfare of Nile Tilapia (Oreochromis sp) Cultured Indoor Tank using BioFloc Tehnology (BFT). Presentation in Aquaculture 2007, 26 February - 3 March 2007. Sna Antonio, Texas, USA

Azim, M.E. Little, D.C. (2008). The biofloc technology (BFT) in indoor tanks: water quality, biofloc composition, and growth and welfare of Nile tilapia (Oreochromis niloticus). Aquaculture. 283, 29-35

Cappuccino, J.G. Sherman, N., (2001), “Microbiology: A Laboratory Manual”. New York: Addison-Wesley Publishing Company

Cowan, S. J. (1974). Cowan and Steel’s Manual for Identification of Medical Bacteria. 2nd Ed. Cambridge: Cambridge University Press

De Schryver, P., Crab, R., Defoirdt, T., Boon, N., Verstraete, W. (2008). The basics of bio-flocss technology: The added value for aquaculture. Aquaculture, 277, 125-137

Dewi, I. M. (2008). Isolasi Bakteri dan Uji Aktifitas Kitinase Termofilik Kasar dari Sumber Air Panas Tinggi Raja, Simalungun, Sumatera Utara. Tersedia dari Tesis. Sekolah Pascasarjana Universitas Sumatera Utara, Medan

Flores, M. L. (2011). The Use of Probiotic In Aquaculture: an overview. International Research Journal of Microbiology, 2, 471-478

Hatmanti, A. (2000). Penganalan Bacillus spp. Oseana, Vol. XXV, No.1, 200, 31-41

Iribarren, D., P. Dagá. M. T. Moreira., G. Feijoo. (2012). Potential environmental effects of probiotics used in aquaculture. Aquacult Int, 20, 779-789

Ismayana, A., Indrasti, N.S., Suprihatin, Akhhiruddin M., Aris.F., (2012). Faktor Rasio C/N Awal dan Laju Aerasi ada Proses CO-Composting Bagasse dan Blotong. Jurnal Teknologi Industri Pertanian, 22 (3), 173-179

Jay, J.M., M.J. Loessner. D.A. Golden. (2005). Modren Food Microbiology Seventh Edition. New York. Springer socience and Business Media. Inc

Lisdayati, Eka. (2013). Potensi Antibakteri Dari Bakteri Asosiasi Lamun (Seagrass) Dari Pulau Bonebatang Perairan Kota Makassar. Tersedia dari Fakultas Ilmu Kelautan Dan Perikanan Universitas Hasanuddin Makassar

Mulyana, D.Y. (2011). Kaya Raya dari Budidaya Ikan dengan Probiotik. Yogyakarta. Berlian media

Putra I, Rusliadi R, Fauzi. (2017). Growth Performance and Feed Unitilization of African Cat Fish Clarias gariepinus Feed Comercial Diet Reared the Biofloc System Enchanced with Probiotic

Putra, S.J. W., Nitisupardjo. M., and Widyorini. N,. (2014). Analisis Hubungan Bahan Organik Dengan Total Bakteri Pada Tambak Udang Intensif Sistem Semibioflok di BBPBAP Jepara. Diponegoro Journal of Maquares, 3, 121-129

Sarbaini. (2015). Isolasi Bakteri Kandidat Probiotik dari Usus Ikan Nila (Oreochromis niloticus) untuk Pengendalian Streptococcus agalactiae. Tersedia dari Pekanbaru: Universitas Riau

Sopian, A., Ikhsan K., Fajar Anggraeni. (2013). Pemanfaatan Bioflok dari Media Pendederan untuk Pemeliharaan Larva Udang Galah (Macrobrachuim rosenbergii). Widyariset, 16 (2), 277-232

Syulasmi, A., Hamdiyati, Y. Kusnadi. (2005). Petunjuk Praktikum Mikrobiologi

Wijaya, C. D. (2014). Identifikasi Dan Uji Aktivitas Antimikroba Bakteri Asam Laktat Yang Diisolasi Dari Rebung Kuning Bambu Betung Yang Difermentasi Pada Suhu 300 C. Tersedia dari Skripsi. Universitas Katolik Soegijapranata Semarang

Willey, J.M., Sherwood, L.M., & Woolverton, C.J., (2009), Prescott’s Principles Of Microbiology. Boston: McGraw-Hill Higher Education




DOI: http://dx.doi.org/10.31258/terubuk.47.1.83-92

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