CHARACTERISTICS AND EFFECTIVENESS OF CHITOSAN ORININATED FROM THE CARAPACE OF T. ANOMALA SHRIMP AS AN ANTIBACTERIAL COMPOUND

Tengku Muhammad Ghazali, Rahman Karnila, Dewita Dewita

Abstract


This study aims to investigate the inhibition zones of the chitosan against Pseudomonas aeruginosa. Carapace from T. anomala is used as raw material of chitosan. There were 5 treatments used, namely positive control (K0+), negative control (K0-), 5% chitosan (K1), 7% chitosan (K2) and 9% (K3) chitosan. The carapace was taken and then washed, dried, refined, demineralization, de-preoteinated, deasitelation and was tested using double layer method, discs and well diffusion. The proximate carapace test results were as follows, water content was 68.18 % gross wight (gw), moisture content 11.77 % dry wight (dw) protein content, 2.35 %dw fat content, 84.43 %dw ash content and carbohydrate content was 1.45 %dw. The chitosan of T. anomala characteristics were yellowish white flakes, odorless and tasteless with yield value, moisture content, ash content, mineral content (Ca, Fe, K, Na, P) and the degree of deacetylation 27.51 %; 7.31 %gw; 3.40 %dw; 12.17 mg/L; 4.90 mg/L; 3.40 mg/L; 5.49 mg/L; 0.27 mg/L; and 73.43 % respectively , Results on antimicrobial sensivity tests using double layer, disk blank and well diffusion. Shown clear zone, 13.87 mm, 11.34 mm and 14.62 mm. these values indicate that the antimicrobial effect of the chitosan is strong.

Keywords


Thalassina anomala; citosan; resistor zone; bacteria

Full Text:

PDF

References


Dewita, Syahrul, Desmelati. (2018). Functional characteristics of cookies containing snakehead (Ophiocephalus striatus) fish protein concentrate fortified with Chlorella sp. International J. of Oceans and Oceanography, 12, 43-52

Farouk, A.E., Faizal A.H.G., Ridzwan B.H.. (2007). New Bacterial Species Isolated from Malaysian Sea Cucumbers with Optimized Secreted Antibacterial Activity. American Journal of Biochemistry and Biotechnology, 64-69

Garriga M, Aymerich HM, Monfort JM. (1993). Bacteriocinogenic activity of Lactobacilli from fermentor sausages. Journal of Applied Bacteriology. 75, 142-148

Hutabarat, M A A, Sari, N I, Leksono T. (2016). Uji Efektivitas Antibakteri Ekstrak Rumput Laut (Eucheuma Cottonii) Terhadap Bakteri Bacillus Cereus dan Pseudomonas aeruginosa. Jurnal Perikanan,1-9

Jay, J. M. (2005). Modern Food Microbiology. Sevent Edition. p: 101-120. USA: Springer Science

Lesbani, A, Yususf, S, Melviani, R, A, M. Karakterisasi Kitin dan Kitosan dari Cangkang Kepiting Bakau (Scylla Serrata). Jurnal penelitian sains, 14, 3(C) 14307

Mayasari, Evita (2006). Pseudomonas aeruginosa; Karakteristik, Infeksi, dan Penanganan. 2006. Dari: http :// library.usu.ac.id

Nursyirwani., W. Asmara,A.E.T.H. Wahyuni dan Triyatno. (2011). Isolasi Bakteri Asam Laktat dari Usus Ikan Kerapu Macan (Epinephelus fuscoguttatus) dan Potensinya Sebagai antivibrio. Ilmu Kelautan, 16(2), 70-77

Pelczar, M. J., E. C. S Chan. (1998). Dasar-dasar Mikrobiologi. Jakarta: UI-Press

Protan Lab. (1987). Cation Polymer for Recovery Valuable by Products from Processing Waste. Burgess

Purnawan, C., (2008). Kitosan dari Cangkang Udang dan Aplikasi Kitosan sebagai Bahan Antibakteri pada Kain Katun. Tersedia dari Thesis. Universitas Gadjah Mada, Yogyakarta

Purwani, E., Retnaningtyas, Dyah W. (2008). Pengembangan Pengawet Alami dari Ekstrak Lengkuas, Kunyit, dan Jahe pada Daging dan Ikan Segar. Tersedia dari Laporan penelitian Fakultas Ilmu Kedokteran Universitas Muhammadiyah, Surakarta

Purwatinigsih, S., Wukirsari, T., Sjahriza, A., Wahyono, D. (2009). Sumber Biomaterial Masa Depan. Bogor: IPB Press

Sudarno, F., A. Setiorini dan H. Suprapto. (2011). Efektivitas Ekstrak Daun Meniran (Phyllantus nururi) sebagai Antibakteri Edwarsiella tarda secara In Vitro. Fakultas perikanan dan Kelautan Universitas Airlangga, 3 (1), 103–108

Suherman B, (2018). Potensi Kitosan Kulit Udang Vannemei (Litopenaeus vannamei) Sebagai Antibakteri Terhadap Staphylococccus epidermidis, Pseudomonas aeruginosa, Propionibacterium agnes, dan Escherichia coli dengan Metode Difusi Cakram Kertas. Tersedia dari Universitas Indonesia Timur Makassar

Suptijah P. (2004). Tingkatan kualitas kitosan hasil modifikasi proses produksi. Jurnal Teknologi Hasil Perairan, 7 (1), 56-67

Syukron, F., R. Karnila dan B. Hasan (2016). Karakteristik Glukosamin Hidrokloroda (HCl GlcN) dari Kitin dan Kepiting Chitosan Biru Kolam (Portunus pelagicus). Tersedia dari Berkala Perikanan Terubuk. ISSN 0126–4265




DOI: http://dx.doi.org/10.31258/terubuk.47.1.93-101

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Fakultas Perikanan dan Kelautan, Universitas Riau

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.