IDENTIFICATION OF GROUPER (EPINEPHELUS SP) AT MUARA ANGKE TRADITIONAL FISH MARKET IN NORTH JAKARTA USING MORPHOLOGY AND DNA BARCODING METHODS

Shodikin Aznardi, Hawis Madduppa

Abstract


Grouper fish is the most exploited commodity for coral fisheries. The controversy that occurred between researchers regarding the identification of fish species is a problem that is quite alarming. Identification using morphometric measurements, shape and color or morphology is considered still not accurate in determining the fish species. The DNA barcoding method uses COI markers by tracing the nucleotide bases to determine which species of biota have advantages in species identification. This study aims to identify grouper fish species in the traditional Muara angke fish market by using morphological methods and DNA barcoding. The identification results obtained from the morphological methods (shape, color and morphometrics) that the sample species are white-tailed grouper fish (Epinephelus areolatus) by looking at morphological characteristics, namely the white lines on the caudal fins and brown spots on the body. Identification using the DNA Barcoding method using the COI markers showed that the fish samples were 95% similar to E. areolatus.

Keywords


morphology identification ; DNA barcoding ; Epinephelus

Full Text:

PDF

References


Allen G, Roger S, Paul H, and Ned D (2003). Reef Fish Identification - Tropical Pacific. New World Publications 2003, Florida. 482 Pages

Fitriadi A.F, R. Elvyra dan Yusfiati (2012). Morfometrik dan Meristik ikan parang – parang (Chirocentrus dorab Forsskal, 1775) di perairan Bengkalis. Biologi, Universitas Riau.

Herbert, P.D.N., Ratnasingham, S. & de Waard JR. (2003). Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society, 270B: 96–99.

Hseu, J.R., Huang, W.I. & Yeong, T.C. (2007). What causes cannibalization-associated suffocation in cultured brown-marbled grouper, Epinephelus fuscoguttatus (Forsskal, 1775). Aquaculture Research, 38: 1056–1060.

Kamal M.M, Agus A.H, Nurlisa A.B, Yulia F dan Rika A. (2019). Autentikasi spesies ikan Kerapu berdasarkan marka gen MT-COI dari perairan Peukan Bada, Aceh. Jurnal Biologi Tropis. 19(2) : 116 – 123

Kim M.J and C.B. Song (2010). First Record of Epinephelus areolatus (Perciformes : Serranidae) from Korea. The Korean Society of Fisheries and Aquatic Science. 13(4) : 340 – 342

KKP. (2012). Statistik perikanan budidaya 2011. Dirjen Perikanan Budidaya, Kementerian Kelautan dan Perikanan Indonesia, Jakarta. 79 halaman.

KKP. (2017). Statistik perikanan budidaya 2016. Dirjen Perikanan Budidaya, Kementerian Kelautan dan Perikanan Indonesia, Jakarta. 84 halaman.

Kumar, S., Stecher, G., Tamura, K., (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol. 33(7):1870-4.

Lakra W.S, M.S Verma, M. Goswami, K.K.Lal, V.Mohindra, P. Punia, A. Gopalakhrisnan, K.V. Singh, R.D. Ward and P. Herbert. (2011). DNA Barcoding Indian Marine Species. Molecular Ecology Resources, 11 : 60-71

WWF, (2015). Perikanan Kerapu dan Kakap : Panduan Penangkapan dan Penanganan. World Wild Fund for Nature Indonesia, Jakarta Selatan. 20 halaman.

www.ncbi.nlm.nih.gov. Nucleotide blast (11 november 2019)




DOI: http://dx.doi.org/10.31258/terubuk.48.1.298-303

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Fakultas Perikanan dan Kelautan, Universitas Riau

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.