EFFECT BIOFLOC SYSTEM AND PROBIOTICS Bacillus sp. D2.2 THAT NON SPECIFIC IMMUNE RESPONSE OF TILAPIA Oreochromis niloticus (Linn, 1758) INFECTED BY Aeromonas hydrophila

Triga Royana Sagala, Supono Supono, Esti Harpeni

Abstract


Disease in tilapia is caused by Aeromonas hydrophila. The disease has an effect on reduction of production cultivation and economic losses. The control efforts taken are to improve the non specific immune response in tilapia cultivated with biofloc system and probiotics against bacterial infection of Aeromonas hydrophila. This study aims to determine effect the non specific immune response in tilapia cultivated with biofloc system and probiotics against bacterial infection of Aeromonas hydrophila infection. The design used in this study completely randomized design with four treatments, control, biofloc, probiotics in feed and a combination of feed probiotics and biofloc. The parameters observed included total leukocytes, differential leukocyte, survival rate, relative percent survival, and water quality. The results showed that cultivated tilapia with bioflok, probiotics, and a combination of both provides a significant immune response to the parameters of total leukocytes and survival rate compared to control and immune response increases after injected bacteria Aeromonas hydrophila.

Keywords


Tilapia; Aeromonas hydrophila; probiotics; biofloc; non specific immune response

Full Text:

PDF

References


Affandi & Tang, U.M. (2002). Fisiologi hewan air. Universitas Riau Press. Riau. 217 hlm.

Amend, D. F. (1981). Potency Testing of Fish Vaccines. Development in Biological Standardization, 49: 447-454.

Anderson, D.P & Siwick, A. (2017). Basic Hematology and Serology for Fish Health Programs. Manila: Asia Fisheries Society. 96 pp.

Aryoseto, L. (2009). Hubungan antara Jumlah Lekosit dengan Morfologi Spermatozoa pada Pasien Infertilitas di Rumah Sakit Dokter Kariadi. Universitas Diponegoro. Semarang.

Badjoeri, M., danWidiyanto.T. 2008. Penggunaan Bakteri Nitrifikasi untuk Bioremediasi dan Pengaruhnya terhadap Konsentrasi Amoniak dan Nitrit di Tambak Udang. Pusat Penelitian Limnologi -LIP1.

Ballester, E. L. C., Abreu, P. C., Cavalli, R. O., Emerenciano, M., De Abreu, L., & Wasielesky Jr, W. (2010). Effect of practical diets with different protein levels on the performance of Farfantepenaeus paulensis juveniles nursed in a zero exchange suspended microbial flocs intensive system. Aquaculture Nutrition, 16(2): 163-172.

BSNI (Badan Standarisasi Nasional Indonesia). (2009). Produksi ikan nila (Oreocrhomis niloticus Bleeker) kelas pembesaran di kolam air tenang. SNI 7550:2009. Jakarta.

Dangeubun, J.L. & Metungun, C. (2017). Hematology of Vibrio alginolyticus-Infected Humpback Grouper Cromileptes altivelis, Under Treatment of Alstonia acuminata Shoot Extract. AACL Bioflux, 10(2), 274-284.

De Schryver, P., Crab, R., Defoirdt, T., Boon, N., & Verstraete, W. (2008). The basics of bioflocs technology: the added value for aquaculture. Aquaculture, 277(3-4): 125-137.

De Schryver, P., Sinha, A.K., Kunwarr PS, Baruah., K, Verstraete, W., Boon, N., De Boeck, G., & Bossier, P. (2010). Poly-β-hydroxybutyrate (PHB) increases growth performance & intestinal bacterial range-wighted richness in juvenile European sea bass, Dicentrarchus labrax. Applied microbiology and biotechnology, 86(5): 1535-1541.

Guyton, A. C., & Hall, J. E. (1997). Buku Ajar Fisiologi Kedokteran Edisi ke 9. Penerbit buku kedokteran EGC.

Hardi, E. H. (2011). Kandidat vaksin potensial Streptococcus agalactiae untuk pencegahan penyakit Streptococcus pada ikan nila (Oreochromis niloticus). Disertasi. Sekolah Pasca Sarjana, Institut Pertanian Bogor, Bogor.

Harikrishan, R., Balasundaram, C., & Heo, M. S. (2010). Effect of probiotics enriched diet on Paralichthys olivaceus infected with lymphocystis disease virus (LCDV). Fish & Shellfish Immunology, 29(5): 868-874.

Hartika, R., Mustahal, M., & Putra, A. N. (2014). Gambaran darah ikan nila (Oreochromis niloticus) dengan penambahan dosis prebiotik yang berbeda dalam pakan. Jurnal Perikanan dan Kelautan, 4(4).

Johnny, F., Zafran, Z., Roza, D., & Mahardika, K. (2017). Hematologi beberapa spesies ikan laut budidaya. Jurnal Penelitian Perikanan Indonesia. 9(4): 63-71.

Kumar, V. & Sharma, A. (2010). Neutrophils: Cinderella of innate immune system. International Immunopharmacology, 10, 1.325–1.334.

Mones, R. A. (2008). Gambaran Darah pada Ikan Mas (Cyprinus carpio Linn) Strain Majalaya yang Berasal dari Daerah Ciampea Bogor. Skripsi. Fakultas Kedokteran Hewan Institut Pertanian Bogor: Bogor.

Pal, G.K. & Pal, P. (2006). Textbook of Practical Physiology. Orient Longman Private Limited. Hyderabat.

Parenrengi, A., Tenriulo, A., & Tampangallo, B. R. (2013). Uji tantang udang windu Paneus monodon transgenis menggunakan bakteri patogen Vibrio harveyi. Konferensi Akuakultus Indonesia. Balai Penelitian dan Pengembangan Budidaya Air Payau Maros, Sulawesi Selatan.

Setianingsih, F. (2018). Efektivitas bakteri probiotik Bacillus sp. D2.2 dan ekstrak tepung ubi jalar sebagai sinbiotik terhadap performa pertumbuhan ikan nila (Oreochromis niloticus). Skripsi. Universitas Lampung.

Setyawan, A., Harpeni, E., Ali, M., Mariska, D. C., & Aji, M. B. (2014). Potensi Agen Bakteri Biokontrol Indigenous Tambak Tradisional Udang Windu (Penaues monodon) di Lampung Timur Strain D2.2, Terhadap Bakteri Patogen Pada Udang dan Ikan. Prosiding Pertemuan Ahli Kesehatan Ikan 2014, Serang 11-13 Februari 2014.

Suguna, P., Binuramesh, C., Abirami, P., Saranya, V., Poornima, K., Rajeswari, V., & Shenbagarathai, R. (2014). Immunostimulation by poly-β hydroxybutyrate–hydroxyvalerate (PHB–HV) from Bacillus thuringiensisin Oreochromis mossambicus. Fish and Shellfish Immunology. 36(1): 90-97.

Sukenda, L. Jamal, D. Wahyuningrum, & A. Hasan. (2008). Penggunaan Kitosan untuk Pencegahan Infeksi Aeromonas hydrophila pada Ikan Lele Dumbo Clarias sp. Jurnal Akuakultur Indonesia, 2: 159-169.

Utami, D. T., Prayitno, S. B., Hastuti, S., & Santika, A. (2013). Gambaran parameter Hematologis pada ikan nila (Oreochromis niloticus) yang diberi vaksin DNA Streptococcus iniae dengan dosis yang berbeda. Journal of Aquaculture Management and Technology, 7-20.

Vonti, O. (2008). Gambaran Darah Ikan Mas (Cyprinus carpio Linn) Strain Sinyonya Yang Berasal dari Daerah Ciampea-Bogor. Skripsi. Fakultas Kedokteran Hewan Institut Pertanian Bogor: Bogor.

Wang, Y. C., Chang, P. S., & Chen, H. Y. (2008). Differential time-series expression of immune-related genes of Pacific white shrimp Litopenaeus vannamei in response to dietary inclusion of β-1, 3-glucan. Fish & Shellfish Immunology, 24(1): 113-121.

Wardoyo, E.W. 2007. Ternyata Ikan Nila, Oreochromis niloticus mempunyai Pontensi Yang Besar Untuk Dikembangkan. Media Akuakultur, Balai Riset Perikanan Budidaya Air Tawar, Bogor, 2(1): 147:150.

Wijayanti, A., Harpeni, E., & Wardiyanto. (2017). Efektivitas Pemberian Bakteri Probiotik Bacillus Sp. D2.2 Dan Ekstrak Ubi Jalar Sebagai Sinbiotik Terhadap Serangan Bakteri Vibrio harveyi pada Udang Vaname (Litopenaeus vannamei). Skripsi. Universitas Lampung. Lampung.




DOI: http://dx.doi.org/10.31258/terubuk.48.1.309-319

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Triga Royana Sagala, Supono Supono, Esti Harpeni

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.